![]() ![]() Both quantitative and qualitative information can be gained through affinity electrophoresis. The Cutting Edge of Affinity Electrophoresis TechnologyĪffinity electrophoresis is an important technique that is widely used to separate and analyze biomolecules in the fields of biology and medicine. Here, we describe advances in analytical techniques involving affinity electrophoresis that have appeared during the last five years. Such methods have recently contributed to profiling of major posttranslational modifications of proteins, such as glycosylation or phosphorylation. Some of these strategies have similarities to affinity chromatography, in that they use a probe or ligand immobilized on a supported matrix for electrophoresis. The various strategies have also been used in the estimation of association/disassociation constants. Hydrophobic membrane proteins can be identified by means of a shift in the mobility induced by a charged detergent. Nucleic acid fragments can be characterized through their affinity to other molecules, for example transcriptional factor proteins. These strategies are based on changes in the electrophoretic patterns of biological macromolecules that result from interactions or complex-formation processes that induce changes in the size or total charge of the molecules. Affinity electrophoresis can be applied through a variety of strategies, such as mobility shift electrophoresis, charge shift electrophoresis or capillary affinity electrophoresis. Kinoshita, Eiji Kinoshita-Kikuta, Emiko Koike, TohruĪffinity electrophoresis is an important technique that is widely used to separate and analyze biomolecules in the fields of biology and medicine. The Cutting Edge of Affinity Electrophoresis Technology. The dependence of the separation resolution on the properties of the sample and its suspension solution was assessed. Biological model materials, hemoglobin and polystyrene latex microspheres, were selected because of their past use as electrophoresis standards and as visible markers for fluid flow due to electroosmosis, spacecraft acceleration or other factors. The second and third flights of the McDonnell Douglas Astronautics Corporation continuous flow electrophoresis system carried samples developed at MSFC intended to evaluate the broad capabilities of free flow electrophoresis in a reduced gravity environment. Highly resolved fractions, however, will only be produced in space because of the sensitivity of this chamber to buoyancy-induced convection in the laboratory. Using a moving wall with a low zeta potential coating, the major sources of flow distortion for an electrophoretic sample stream are removed. A new high resolution apparatus designed for space was built as a laboratory prototype. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |